Identification and in Vitro Evaluation of Antifungal Activity of Alkaloids from Siparuna Sessiliflora Kunth A. Dc. Leaves

Total alkaloid extract of the leaves of Siparuna sessiliflora was analyzed by gas chromatography coupled to a mass detector (GC-MS) and nuclear magnetic resonance (NMR). Ushinsunine and 4'-O-methyl-Nmethylcoclaurine, were identified as major compounds, along with liriodenine, corydine, Nmethyllaurotetanine, boldine, N-methylcoridaldine, nornuciferine, stepholidine and nantenine. The alkaloids ushinsunine, 4'-O-methyl-N-methylcoclaurine and liriodenine were isolated and their identities confirmed by MS and NMR. Antifungal activity of alkaloid total extract was evaluated against Fusarium oxysporum, Alternaria sp. and Aspergillus niger, displaying low antifungal activity against these strains.